The U.S. Department of Agriculture's Animal and Plant Health Inspection Service (APHIS) has again reopened the comment period on the protocols for regulating and deregulating pale cyst nematode (PCN)-infested and associated areas. APHIS initially accepted comments on the protocols March 1, 2019, and again on June 26, 2019 for 30 days. APHIS is providing the public with an additional opportunity to comment on the science supporting the protocols, including the sources of the methods informing their content. In an effort to give all interested parties ample opportunities to comment, we are reopening the comment period for 30 days beginning June 5, 2020 and ending July 6, 2020. Members of the public are encouraged to participate in the development of these protocols by submitting comments starting on the day of publication until July 6, 2020 at: https://www.regulations.gov/docket/APHIS-2018-0041.